"use strict"; const {Fragment: _Fragment, jsx: _jsx, jsxs: _jsxs} = arguments[0]; const {useMDXComponents: _provideComponents} = arguments[0]; function _createMdxContent(props) { const _components = { a: "a", code: "code", h1: "h1", h2: "h2", li: "li", p: "p", path: "path", pre: "pre", span: "span", svg: "svg", ul: "ul", ..._provideComponents(), ...props.components }; return _jsxs(_Fragment, { children: [_jsx(_components.p, { children: "Seurat单细胞分析流程主要就是以下十句代码" }), "\n", _jsx(_components.pre, { className: "language-r", children: _jsxs(_components.code, { className: "language-R code-highlight", children: [_jsxs(_components.span, { className: "code-line", children: ["pbmc.counts ", _jsx(_components.span, { className: "token operator", children: "<-" }), " Read10X", _jsx(_components.span, { className: "token punctuation", children: "(" }), "data.dir ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"data/filtered_gene_bc_matrices/hg19/\"" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["pbmc ", _jsx(_components.span, { className: "token operator", children: "<-" }), " CreateSeuratObject", _jsx(_components.span, { className: "token punctuation", children: "(" }), "counts ", _jsx(_components.span, { className: "token operator", children: "=" }), " pbmc.counts", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["pbmc ", _jsx(_components.span, { className: "token operator", children: "<-" }), " NormalizeData", _jsx(_components.span, { className: "token punctuation", children: "(" }), "object ", _jsx(_components.span, { className: "token operator", children: "=" }), " pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["pbmc ", _jsx(_components.span, { className: "token operator", children: "<-" }), " FindVariableFeatures", _jsx(_components.span, { className: "token punctuation", children: "(" }), "object ", _jsx(_components.span, { className: "token operator", children: "=" }), " pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["pbmc ", _jsx(_components.span, { className: "token operator", children: "<-" }), " ScaleData", _jsx(_components.span, { className: "token punctuation", children: "(" }), "object ", _jsx(_components.span, { className: "token operator", children: "=" }), " pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["pbmc ", _jsx(_components.span, { className: "token operator", children: "<-" }), " RunPCA", _jsx(_components.span, { className: "token punctuation", children: "(" }), "object ", _jsx(_components.span, { className: "token operator", children: "=" }), " pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["pbmc ", _jsx(_components.span, { className: "token operator", children: "<-" }), " FindNeighbors", _jsx(_components.span, { className: "token punctuation", children: "(" }), "object ", _jsx(_components.span, { className: "token operator", children: "=" }), " pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["pbmc ", _jsx(_components.span, { className: "token operator", children: "<-" }), " FindClusters", _jsx(_components.span, { className: "token punctuation", children: "(" }), "object ", _jsx(_components.span, { className: "token operator", children: "=" }), " pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["pbmc ", _jsx(_components.span, { className: "token operator", children: "<-" }), " RunTSNE", _jsx(_components.span, { className: "token punctuation", children: "(" }), "object ", _jsx(_components.span, { className: "token operator", children: "=" }), " pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["DimPlot", _jsx(_components.span, { className: "token punctuation", children: "(" }), "object ", _jsx(_components.span, { className: "token operator", children: "=" }), " pbmc", _jsx(_components.span, { className: "token punctuation", children: "," }), " reduction ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"tsne\"" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] })] }) }), "\n", _jsx(_components.p, { children: "从数据读取开始" }), "\n", _jsxs(_components.h1, { id: "数据", children: [_jsx(_components.a, { className: "heading-link", href: "#数据", children: _jsx(_components.svg, { xmlns: "http:www.w3.org/2000/svg", fill: "#ab2b65", viewBox: "0 0 20 20", className: "w-5 h-5", children: _jsx(_components.path, { fillRule: "evenodd", clipRule: "evenodd", d: "M9.493 2.853a.75.75 0 00-1.486-.205L7.545 6H4.198a.75.75 0 000 1.5h3.14l-.69 5H3.302a.75.75 0 000 1.5h3.14l-.435 3.148a.75.75 0 001.486.205L7.955 14h2.986l-.434 3.148a.75.75 0 001.486.205L12.456 14h3.346a.75.75 0 000-1.5h-3.14l.69-5h3.346a.75.75 0 000-1.5h-3.14l.435-3.147a.75.75 0 00-1.486-.205L12.045 6H9.059l.434-3.147zM8.852 7.5l-.69 5h2.986l.69-5H8.852z" }) }) }), "数据"] }), "\n", _jsxs(_components.h2, { id: "三文件", children: [_jsx(_components.a, { className: "heading-link", href: "#三文件", children: _jsx(_components.svg, { xmlns: "http:www.w3.org/2000/svg", fill: "#ab2b65", viewBox: "0 0 20 20", className: "w-5 h-5", children: _jsx(_components.path, { fillRule: "evenodd", clipRule: "evenodd", d: "M9.493 2.853a.75.75 0 00-1.486-.205L7.545 6H4.198a.75.75 0 000 1.5h3.14l-.69 5H3.302a.75.75 0 000 1.5h3.14l-.435 3.148a.75.75 0 001.486.205L7.955 14h2.986l-.434 3.148a.75.75 0 001.486.205L12.456 14h3.346a.75.75 0 000-1.5h-3.14l.69-5h3.346a.75.75 0 000-1.5h-3.14l.435-3.147a.75.75 0 00-1.486-.205L12.045 6H9.059l.434-3.147zM8.852 7.5l-.69 5h2.986l.69-5H8.852z" }) }) }), "三文件"] }), "\n", _jsx(_components.p, { children: "使用Seurat包进行分析，它能够处理和分析来自不同平台(包括10x Genomics、DroNc-seq、Smart-seq等)的scRNA-seq数据。" }), "\n", _jsxs(_components.p, { children: ["最常见的是从包含", _jsx(_components.code, { className: "language-auto", children: "matrix.mtx" }), "（原始counts），", _jsx(_components.code, { className: "language-auto", children: "barcodes.tsv" }), "（细胞信息），", _jsx(_components.code, { className: "language-auto", children: "genes.tsv" }), "（基因信息）三个文件的文件夹读取数据。"] }), "\n", _jsx(_components.pre, { className: "language-r", children: _jsxs(_components.code, { className: "language-R code-highlight", children: [_jsxs(_components.span, { className: "code-line", children: ["xxx.data ", _jsx(_components.span, { className: "token operator", children: "<-" }), " Read10X", _jsx(_components.span, { className: "token punctuation", children: "(" }), "data.dir ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"data/\"" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["xxx ", _jsx(_components.span, { className: "token operator", children: "<-" }), " CreateSeuratObject", _jsx(_components.span, { className: "token punctuation", children: "(" }), "xxx.data", _jsx(_components.span, { className: "token punctuation", children: "," }), " project ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"name\"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), " min.cells ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token number", children: "3" }), _jsx(_components.span, { className: "token punctuation", children: "," }), " min.features ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token number", children: "200" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), " ", _jsx(_components.span, { className: "token comment", children: "# mincell为初步过滤，基因至少在3个细胞中表达；minfeature指细胞至少表达多少个基因才会被保留。" }), "\n"] })] }) }), "\n", _jsxs(_components.ul, { children: ["\n", _jsx(_components.li, { children: "barcodes.tsv 和 genes.tsv，就是表达矩阵的行名和列名；" }), "\n", _jsx(_components.li, { children: "matrix.mtx 文件是3列，第一列是行号，第二列是列号，第三列是基因表达量，而且仅仅是列出有表达量的基因即可，即仅仅是显示非0值。" }), "\n"] }), "\n", _jsx(_components.pre, { className: "language-r", children: _jsxs(_components.code, { className: "language-R code-highlight", children: [_jsxs(_components.span, { className: "code-line", children: [_jsx(_components.span, { className: "token operator", children: "$" }), "head barcode.tsv \n"] }), _jsxs(_components.span, { className: "code-line", children: ["AAACATACAACCAC", _jsx(_components.span, { className: "token operator", children: "-" }), _jsx(_components.span, { className: "token number", children: "1" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["AAACATTGAGCTAC", _jsx(_components.span, { className: "token operator", children: "-" }), _jsx(_components.span, { className: "token number", children: "1" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["AAACATTGATCAGC", _jsx(_components.span, { className: "token operator", children: "-" }), _jsx(_components.span, { className: "token number", children: "1" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["AAACCGTGCTTCCG", _jsx(_components.span, { className: "token operator", children: "-" }), _jsx(_components.span, { className: "token number", children: "1" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["AAACCGTGTATGCG", _jsx(_components.span, { className: "token operator", children: "-" }), _jsx(_components.span, { className: "token number", children: "1" }), "\n"] }), _jsx(_components.span, { className: "code-line", children: "\n" }), _jsxs(_components.span, { className: "code-line", children: [_jsx(_components.span, { className: "token operator", children: "$" }), "head genes.tsv\n"] }), _jsxs(_components.span, { className: "code-line", children: ["ENSG00000243485\tMIR1302", _jsx(_components.span, { className: "token operator", children: "-" }), _jsx(_components.span, { className: "token number", children: "10" }), "\n"] }), _jsx(_components.span, { className: "code-line", children: "ENSG00000237613\tFAM138A\n" }), _jsx(_components.span, { className: "code-line", children: "ENSG00000186092\tOR4F5\n" }), _jsxs(_components.span, { className: "code-line", children: ["ENSG00000238009\tRP11", _jsx(_components.span, { className: "token operator", children: "-" }), _jsx(_components.span, { className: "token number", children: "34P13" }), _jsx(_components.span, { className: "token number", children: ".7" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["ENSG00000239945\tRP11", _jsx(_components.span, { className: "token operator", children: "-" }), _jsx(_components.span, { className: "token number", children: "34P13" }), _jsx(_components.span, { className: "token number", children: ".8" }), "\n"] }), _jsx(_components.span, { className: "code-line", children: "\n" }), _jsxs(_components.span, { className: "code-line", children: [_jsx(_components.span, { className: "token operator", children: "$" }), "head matrix.mtx\n"] }), _jsxs(_components.span, { className: "code-line", children: [_jsx(_components.span, { className: "token percent-operator operator", children: "%%" }), "MatrixMarket matrix coordinate real general\n"] }), _jsx(_components.span, { className: "code-line", children: "%\n" }), _jsxs(_components.span, { className: "code-line", children: [_jsx(_components.span, { className: "token number", children: "32738" }), " ", _jsx(_components.span, { className: "token number", children: "2700" }), " ", _jsx(_components.span, { className: "token number", children: "2286884" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [_jsx(_components.span, { className: "token number", children: "32709" }), " ", _jsx(_components.span, { className: "token number", children: "1" }), " ", _jsx(_components.span, { className: "token number", children: "4" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [_jsx(_components.span, { className: "token number", children: "32707" }), " ", _jsx(_components.span, { className: "token number", children: "1" }), " ", _jsx(_components.span, { className: "token number", children: "1" }), "\n"] })] }) }), "\n", _jsxs(_components.h2, { id: "仅有一个稀疏矩阵", children: [_jsx(_components.a, { className: "heading-link", href: "#仅有一个稀疏矩阵", children: _jsx(_components.svg, { xmlns: "http:www.w3.org/2000/svg", fill: "#ab2b65", viewBox: "0 0 20 20", className: "w-5 h-5", children: _jsx(_components.path, { fillRule: "evenodd", clipRule: "evenodd", d: "M9.493 2.853a.75.75 0 00-1.486-.205L7.545 6H4.198a.75.75 0 000 1.5h3.14l-.69 5H3.302a.75.75 0 000 1.5h3.14l-.435 3.148a.75.75 0 001.486.205L7.955 14h2.986l-.434 3.148a.75.75 0 001.486.205L12.456 14h3.346a.75.75 0 000-1.5h-3.14l.69-5h3.346a.75.75 0 000-1.5h-3.14l.435-3.147a.75.75 0 00-1.486-.205L12.045 6H9.059l.434-3.147zM8.852 7.5l-.69 5h2.986l.69-5H8.852z" }) }) }), "仅有一个稀疏矩阵"] }), "\n", _jsx(_components.pre, { className: "language-r", children: _jsxs(_components.code, { className: "language-R code-highlight", children: [_jsxs(_components.span, { className: "code-line", children: ["matrix_data ", _jsx(_components.span, { className: "token operator", children: "<-" }), " read.table", _jsx(_components.span, { className: "token punctuation", children: "(" }), _jsx(_components.span, { className: "token string", children: "\"single_cell_datamatrix.txt\"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), " sep", _jsx(_components.span, { className: "token operator", children: "=" }), _jsx(_components.span, { className: "token string", children: "\"\\t\"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), " header", _jsx(_components.span, { className: "token operator", children: "=" }), "T", _jsx(_components.span, { className: "token punctuation", children: "," }), " row.names", _jsx(_components.span, { className: "token operator", children: "=" }), _jsx(_components.span, { className: "token number", children: "1" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["dim", _jsx(_components.span, { className: "token punctuation", children: "(" }), "matrix_data", _jsx(_components.span, { className: "token punctuation", children: ")" }), " ", _jsx(_components.span, { className: "token comment", children: "# 获取矩阵的维数（x行y列），y为细胞数" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["seurat_obj ", _jsx(_components.span, { className: "token operator", children: "<-" }), " CreateSeuratObject", _jsx(_components.span, { className: "token punctuation", children: "(" }), "counts ", _jsx(_components.span, { className: "token operator", children: "=" }), " matrix_data", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] })] }) }), "\n", _jsxs(_components.h2, { id: "rds文件", children: [_jsx(_components.a, { className: "heading-link", href: "#rds文件", children: _jsx(_components.svg, { xmlns: "http:www.w3.org/2000/svg", fill: "#ab2b65", viewBox: "0 0 20 20", className: "w-5 h-5", children: _jsx(_components.path, { fillRule: "evenodd", clipRule: "evenodd", d: "M9.493 2.853a.75.75 0 00-1.486-.205L7.545 6H4.198a.75.75 0 000 1.5h3.14l-.69 5H3.302a.75.75 0 000 1.5h3.14l-.435 3.148a.75.75 0 001.486.205L7.955 14h2.986l-.434 3.148a.75.75 0 001.486.205L12.456 14h3.346a.75.75 0 000-1.5h-3.14l.69-5h3.346a.75.75 0 000-1.5h-3.14l.435-3.147a.75.75 0 00-1.486-.205L12.045 6H9.059l.434-3.147zM8.852 7.5l-.69 5h2.986l.69-5H8.852z" }) }) }), "RDS文件"] }), "\n", _jsxs(_components.p, { children: ["rds是二进制文件，直接用", _jsx(_components.code, { className: "language-auto", children: "readRDS" }), "函数读取"] }), "\n", _jsx(_components.pre, { className: "language-r", children: _jsxs(_components.code, { className: "language-R code-highlight", children: [_jsxs(_components.span, { className: "code-line", children: ["rm", _jsx(_components.span, { className: "token punctuation", children: "(" }), "list ", _jsx(_components.span, { className: "token operator", children: "=" }), " ls", _jsx(_components.span, { className: "token punctuation", children: "(" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["pbmc ", _jsx(_components.span, { className: "token operator", children: "<-" }), " readRDS", _jsx(_components.span, { className: "token punctuation", children: "(" }), _jsx(_components.span, { className: "token string", children: "\"panc8.rds\"" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["saveRDS", _jsx(_components.span, { className: "token punctuation", children: "(" }), "pbmc", _jsx(_components.span, { className: "token punctuation", children: "," }), _jsx(_components.span, { className: "token string", children: "\"pbmc.rds\"" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] })] }) }), "\n", _jsx(_components.pre, { className: "language-r", children: _jsxs(_components.code, { className: "language-R code-highlight", children: [_jsxs(_components.span, { className: "code-line", children: ["str", _jsx(_components.span, { className: "token punctuation", children: "(" }), "pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [_jsx(_components.span, { className: "token punctuation", children: "(" }), "out ", _jsx(_components.span, { className: "token operator", children: "<-" }), " capture.output", _jsx(_components.span, { className: "token punctuation", children: "(" }), "str", _jsx(_components.span, { className: "token punctuation", children: "(" }), "pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: ["out2 ", _jsx(_components.span, { className: "token operator", children: "<-" }), " str", _jsx(_components.span, { className: "token punctuation", children: "(" }), "pbmc", _jsx(_components.span, { className: "token punctuation", children: ")" }), " ", _jsx(_components.span, { className: "token percent-operator operator", children: "%>%" }), " \n"] }), _jsxs(_components.span, { className: "code-line", children: [" capture.output", _jsx(_components.span, { className: "token punctuation", children: "(" }), ".", _jsx(_components.span, { className: "token punctuation", children: ")" }), " ", _jsx(_components.span, { className: "token percent-operator operator", children: "%>%" }), " \n"] }), _jsxs(_components.span, { className: "code-line", children: [" gsub", _jsx(_components.span, { className: "token punctuation", children: "(" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" pattern ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"\\\\.\\\\. \"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" replacement ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"#\"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" x ", _jsx(_components.span, { className: "token operator", children: "=" }), " .\n"] }), _jsxs(_components.span, { className: "code-line", children: [" ", _jsx(_components.span, { className: "token punctuation", children: ")" }), " ", _jsx(_components.span, { className: "token percent-operator operator", children: "%>%" }), " \n"] }), _jsxs(_components.span, { className: "code-line", children: [" gsub", _jsx(_components.span, { className: "token punctuation", children: "(" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" pattern ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"\\\\.\\\\.@\"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" replacement ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"# \"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" x ", _jsx(_components.span, { className: "token operator", children: "=" }), " .\n"] }), _jsxs(_components.span, { className: "code-line", children: [" ", _jsx(_components.span, { className: "token punctuation", children: ")" }), " ", _jsx(_components.span, { className: "token percent-operator operator", children: "%>%" }), " \n"] }), _jsxs(_components.span, { className: "code-line", children: [" gsub", _jsx(_components.span, { className: "token punctuation", children: "(" }), "pattern", _jsx(_components.span, { className: "token operator", children: "=" }), _jsx(_components.span, { className: "token string", children: "\"^\\\\s+#\"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), "replace", _jsx(_components.span, { className: "token operator", children: "=" }), _jsx(_components.span, { className: "token string", children: "\"#\"" }), _jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] }), _jsx(_components.span, { className: "code-line", children: "\n" }), _jsxs(_components.span, { className: "code-line", children: ["mindr", _jsx(_components.span, { className: "token operator", children: "::" }), "mm", _jsx(_components.span, { className: "token punctuation", children: "(" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" from ", _jsx(_components.span, { className: "token operator", children: "=" }), " out2", _jsx(_components.span, { className: "token punctuation", children: "," }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" input_type ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"markdown\"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" output_type ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"widget\"" }), _jsx(_components.span, { className: "token punctuation", children: "," }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [" root ", _jsx(_components.span, { className: "token operator", children: "=" }), " ", _jsx(_components.span, { className: "token string", children: "\"Seurat\"" }), "\n"] }), _jsxs(_components.span, { className: "code-line", children: [_jsx(_components.span, { className: "token punctuation", children: ")" }), "\n"] })] }) })] }); } function MDXContent(props = {}) { const {wrapper: MDXLayout} = { ..._provideComponents(), ...props.components }; return MDXLayout ? _jsx(MDXLayout, { ...props, children: _jsx(_createMdxContent, { ...props }) }) : _createMdxContent(props); } return { default: MDXContent };